Expression of DOC2, Rasp21 and C-Fos Proteins in Colorectal ...

Abstract

Background and ObjectivesThe colorectal cancer is one of the most common gastrointestinal tumors.With the changing habit of people?s life in recent years, the incidence of colorectal cancer in China shows an upward trend.At present,the most effective treatment is still surgical removal of tumor as much as possible.Colorectal cancer is always diagnosed in advanced stage because there is no symptom in early period.The majority of patients die of local recurrence or distant metastasis although the primary tumor has been resected.The survival rate of colorectal cancer is greatly related to lymph node and distant metastasis. However,the formation mechanism of the metastasis is not yet very clear.Although the comprehensive.treatment effect of colorectal cancer has been made great progress in recent years,the treatment effect of patients with metastasis is still not optimistic. Early diagnosis and anticipation the risk of metastasis are very critical to improve the prognosis of colorectal cancer patients. So,it is very important for us to find a meaningful pathology indicators which can indicate the evolution and prognosis of colorectal cancer.Along with the development of science and technology,more and more people recognize that the occurrence of colorectal cancer is a multi-stage,multi-step,complex process and is related to abnormal changes of multiple genes.The Human DOC2 belongs to Disabled gene family,which is also named the Dab2.DOC2/Dab2?s structure suggest that it may be a signal molecule,which can not only regulate the growth and differentiation,but also have the function of tumor suppression.The specific mechanism of DOC2/Dab2 is not entirely clear,so the tumor suppressor gene has not yet formed a conclusion.Oncogene has become a hotspot in recent years.Ras genes are more closely-watched cancer gene in current study,the activation of which is closely related with a variety of tumor.RasP21 is most closely related to the occurrence of colorectal cancer.lt not only participates in cell malignant transformation through abnormal signal transduction,but also plays an important role in tumor angiogenesis.So,the role of Ras and its related signal transduction pathways are worthy of further study in colorectal cancer genesis and development.C-Fos is a kind of nuclear fission signal effector protein,which not only plays an important regulatory role in cell proliferation and growth,but also regulates the transcription of multiple genes.The expression and regulation of c-Fos is closely related with the occurrence and development of many diseases.So,in this study we investigate the expression of DOC2/Dab2,rasP21,c-Fos in colorectal cancer and their relationship with clinicopathological parameters in the development process of colorectal cancer. These provide a valuable clinicopathological indicators and new ideas to develop the new tumor target therapy.Materials and Methods1.110 cases of colorectal carcinoma tissue,29 cases of colorectal adenoma tissue and 40 cases of para-carcinoma normal mucosa tissue were collected. Any chemotherapy, radiotherapy and other biological targeted therapy have never been carried out in all cases before surgery.2. The expression of DOC2/Dab2,rasP21 and c-Fos proteins were detected by immunohistochemistry.The correlation among the three indicators and their correlations to the clinicopathologic parameters of colorectal carcinoma were analyzed,and the interaction of the three indicators were explored in colorectal cancer.3. Statistical analysis,SPSS 13.0 software was used for statistical analysis.Chi-square test or Fisher?s exact test of probabilities were used to analyze the date.The correlation of DOC2/Dab2,rasP21 and c-Fos was analyzed with Spearman rank correlation.Test leve a=0.05.Results1. Immunohistochemistry results showed:the positive staining of DOC2/Dab2 protein in colorectal tissue was located in the cytoplasm.The positive expression rates of DOC2/Dab2 protein in para-carcinoma normal colorectal tissue, colorectal adenoma and colorectal carcinoma were 82.50%(33/40),55.17%(16/29) and 30.91% (34/110),the difference among the three groups was statistically significant(P 0.05).But in the research we revealed that the expression level was significantly concerned with lymph node and liver metastasis(P0.05).But in the research we found that the expression level was significantly correlated with differentiated degree, lymph node and liver metastasis (P0.05).But the research revealed that the expression level was significantly concerned with differentiated degree (P

Source: http://www.res-medical.com/oncology/55589

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